endobj
Treatment with eugenol at their MIC (0.0125%) and MBC (0.025%) reduced the viability and resulted in complete inhibition of the organism. Eugenol (4-allyl-2-methoxyphenol), is a naturally occurring phenol essential oil extracted from cloves, is known to be an antioxidant (Jirovetz et al., 2006, Ogata et al., 2000), a monoamine oxidase (MAO) inhibitor and known to have neuroprotective effects (Kabuto et al., 2007). STCW Smovod Go Smalo 4. The same is true in any industry. ]eέ�lBW��sO�|i��rMhR�5Wn��0CBrr���w�OB�L_����, +�zz���Y*Z�ߺ������O�m%ԎF��T�RYo���֬��49i��C֯v�jƫ����P?���mr�0��!�Z�!�R\P?~��������gg���f����N�\����� Eugenol inactivated Salmonella typhi within 60 min exposure. The vapors began to travel up into the distilling head and then began to accumulate in the distilling column. 2. The results were obtained (Figure 1) and analyzed to find the major constituent. b. Clove Oil Characterization #is experiment used clove oil taken from MITRA PALA MAS, Kampung Krajan Wanayasa, Purwakarta. 0.7%) and from chemical method (89.6% s.d. We use cookies to help provide and enhance our service and tailor content and ads. Use this table when you already know the frequency of your material. 4 0 obj
Thermo Fisher Scientific. Fig. 426 g – 104. The chemo-attractant property of eugenol combined with the observed high antibacterial activity at alkaline pH favors the fact that the compound can work more efficiently when given in vivo. x��=�r��wE����K���#4�V�Ǟ���ȇ"�/���!�3����WfP,�{XO���D��@�~s�p}1�=�~���o�������ן������x8��py������~�ͷoW�|z���wj�TQV�O/_�U ��U��RW�����ܾ|Q�.����/~^ش�l���W��n��M���v���Y�#U�������}s8lt���'���:m�����#g� _���5���}��/_��w���ڢ�8�?�OrmkU�Q[����Y�亵��F�V���x�.�vm�sm���͡ޕE5n������H����J��RɮZ�]����a�LU4��Uרb��.j-�L�}Ѵ�sYh��ч�)B�yV��J̑��O�d{��-0�_�Z}[�ZN��1F����m_m�r|��sO���)�FBQٶ}�Fl��[!/ʶ$S�1���Ra���o����A��z!^m]���[���P� Access a targeted collection of application notes, case studies, videos, webinars and white papers covering a range of applications for Fourier Transform infrared spectroscopy, Near-infrared spectroscopy, Raman spectroscopy, Nuclear Magnetic Resonance, Ultraviolet-Visible (UV-Vis) spectrophotometry, X-Ray Fluorescence, and more. The measurement of release of 260 nm absorbing intracellular materials, SDS-PAGE, SEM and AFM analysis confirmed the disruptive action of eugenol on cytoplasmic membrane. 0.7%). The distillate was poured into a clean, dry separatory funnel. 000g?100%=21. x��YɎ$5��W��x_$�R�n-q� 8L���OD8�vfeU3M_Z���t��X^��o�{m���i�����A�C҉>����{w�YU��i��j�R��D��>�ZMQ�O�÷Ƹ�1�s����ӃJU�W{��\�{��� w���|�+�]�y��K{��]�H���uy�i��,��1����o�����Q������-������Wf+\�v�*׃ 0 mL of distilled water was obtained and added to the same 500 mL round bottom flask. In health remedies, they find use as treatment oils, pastes and salves for minor aches, pains, congestion and coughing. As seen in Figure 1, TBN changes are indicated in the IR spectra https://doi.org/10.1016/j.jep.2010.04.025. stream
If you do not receive an email within 10 minutes, your email address may not be registered, U��. To evaluate the antibacterial activity of eugenol and its mechanism of bactericidal action against Salmonella typhi. 5. 120 (2010) 1170-1177. The bottom layer of the separatory funnel was added to the same 50 mL flask. The Bunsen burner was lit and then used to “paint” the bottom of the 500 mL round bottom flask with water and ground cloves in it. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. GC and IR spectra of clove oil are shown in Figs 4 and 5 respectively. Eugenol (an essential oil of clove) ... Control samples without eugenol treatment were prepared and subjected to analysis as mentioned above. 356 g. Magnesium sulfate was added to the beaker until the powder stopped clumping together. Which bands in the IR correspond to the functional groups of eugenol? endobj
The purpose of this lab was to obtain a pure sample of clove oil from ground cloves using steam distillation, perform infrared spectroscopy on the sample, and analyze the IR to identify the major constituent in the clove oil. By continuing you agree to the use of cookies. Experimental. (2017, Mar 17). You can get your custom paper from Retrieved from https://graduateway.com/isolation-of-the-major-constituents-of-clove-oil/, This is just a sample. Learn more. The deformation of macromolecules in the membrane, upon treatment with eugenol was verified by FT-IR spectroscopy. �RS���M}Z%c�U۞�y�I��R~M6ʛT�-�Y��`C��>���]3� +���.�&G
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MGX�Õ����T�6�}�U(]�:!��joږ������T 070 g The percent recovery was found using the following equation. The full version of „Nicodom IR Essential Oils“ include common oil name, product type (essential or other), region, latin plant name and other sample information. Result and Discussions 3.1 Chemicals composition analysis of clove leaf oil using GC-MS Fig.
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